[Histonet] Faded H&E tissue section

[Tony Henwood]

I thought he may have reported this in Histologic.
This trick certainly works. You can notice the nuclear clarity on GIT and to a lesser extent skin biopsies stained with PAS.

Regards,

Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired)
Principal Scientist, the Children’s Hospital at Westmead (Retired)
Adjunct Fellow, School of Medicine, University of Western Sydney.

From: Akemi Allison<mailto:akemiat3377 at gmail.com>
Sent: Friday, 10 November 2023 1:30 PM
To: jayalakshmy p.s<mailto:psjayalakshmy at gmail.com>
Cc: Tony Henwood<mailto:afhenwood at outlook.com>; histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Faded H&E tissue section

Hi Tony:
I actually gave that tip to Lee back in 1979 when he came to OHSU to give a seminar to the Oregon Histology Society.  I found out that when I was doing GMA and the pathologists didn’t like Gills Hematoxylin. They loved my PAS on GMA so I tried using 1% Periodic Acid before using Harris Hematoxylin for H&E’s on GMA and it turned out beautifully. Guess he shared my tip with the rest of our society. He and I became closer se friends and we shared several tips, including his Movat’s tips which he didn’t publish, but I shared them in Frieda’s 4th Ed.

Best,
Akemi Allison-Tacha, BS, HT/HTL (ASCP)

Sent from my iPhone

> On Nov 9, 2023, at 6:11 PM, jayalakshmy p.s via Histonet <histonet at lists.utsouthwestern.edu> wrote:
>
> Thanks, I'll check it out.
>
>> On Fri, Nov 10, 2023, 6:58 AM Tony Henwood <afhenwood at outlook.com> wrote:
>>
>> You could treat the decoverslipped section in 1% periodic acid (same as
>> used in the PAS technique)  for 30 minutes prior to H&E staining. This
>> might improve the H&E staining.
>>
>>
>>
>> I believe Lee Luna suggested this but for the life of me, I can’t find the
>> reference!
>>
>>
>>
>> Regards,
>>
>>
>>
>> Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired)
>>
>> Principal Scientist, the Children’s Hospital at Westmead (Retired)
>>
>> Adjunct Fellow, School of Medicine, University of Western Sydney.
>>
>>
>>
>> *From: *jayalakshmy p.s via Histonet <histonet at lists.utsouthwestern.edu>
>> *Sent: *Friday, 10 November 2023 3:43 AM
>> *To: *histonet at lists.utsouthwestern.edu
>> *Subject: *[Histonet] Faded H&E tissue section
>>
>>
>>
>> Hello,
>> I would like to know how to effectively restain a faded H&E tissue section.
>> The color becomes dull when re stained. Somebody please advise.
>> Prof. Jayalakshmy. P S
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>> Histonet mailing list
>> Histonet at lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>>
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