[Histonet] ZInc formalin

[Elizabeth Chlipala]

Hello All

Unless something has changed,  this is how I remember it.   That antibody used to work for mouse on FFPE tissue samples ( I had a lot that did work and it was great, but that was years ago) and then the goat died or something like that??????.   From what I understand now the current lot numbers do not work on FFPE tissues samples.  Has something changed recently that this antibody will work again in FFPE mouse tissues, if so I'm interested.  That antibody worked on multiple species quite well before.

Thanks

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Laboratory Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
Work (303) 682-3949
Fax (303) 682-9060
Cell (303) 881-0763
liz <@t> premierlab.com
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mehmet Fatih BOZKURT
Sent: Wednesday, September 18, 2013 10:41 PM
To: Mesruh Turkekul; Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] ZInc formalin

Santa Cruz, SC-1506 is a good goat polyclonal antibody for mouse tissues.
also there is rabbit IgG that is coded SC-1506R.


On Thu, Sep 19, 2013 at 4:32 AM, Mesruh Turkekul <turkekul <@t> gmail.com> wrote:

> Hi Liz,
>
> I checked the web site and the images of FFPE sections stained with
> CD31 from Dianova. It seems that it mostly stains large blood vessels.
> Which I have experienced with most CD31 Ab on the market. Does this ab
> stain all the capillaries in your experiments?
>
> Thanks again,
> Mesruh
>
> Sent from my iPad
>
> On Sep 18, 2013, at 7:51 PM, Elizabeth Chlipala <liz <@t> premierlab.com>
> wrote:
>
> > Dianova has an excellent antibody for CD31 in mice for FFPE tissue
> samples.
> >
> > Liz
> >
> > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO
> > Box 18592 Boulder, CO 80308
> > (303) 682-3949 office
> > (303) 881-0763 cell
> > (303) 682-9060 fax
> > liz <@t> premierlab.com
> >
> > Ship to address:
> >
> > Premier Laboratory, LLC
> > 1567 Skyway Drive, Unit E
> > Longmont, CO 80504
> > ________________________________________
> > From: histonet-bounces <@t> lists.utsouthwestern.edu [
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mesru T [
> turkekul <@t> gmail.com]
> > Sent: Wednesday, September 18, 2013 4:29 PM
> > To: histonet <@t> lists.utsouthwestern.edu
> > Subject: [Histonet] ZInc formalin
> >
> > Dear Histonetters,
> >
> > I do not think there is a good CD31 (PECAM-1) antibody that will
> > work on FFPE mouse tissues. If you have one please let me know. We
> > use CD34 rat mono (Abcam) and Meca-32 Rat mono (Dev Hybridoma Bank
> > Iowa Univ.) to
> label
> > endothelial cells of blood vessels in FFPE mouse tissues.
> > There is a CD31 Rat mono from BD that will work of 10% NBF fixed,
> > sucrose protected cryo embedded mouse tissues with antigen retrieval.
> > The same CD31 Rat mono from BD will also work on Zinc Fixative  from
> > BD cat# 51-7538KZ ( it is a solution of ZnCl2 and maybe other zinc
> > salts but no aldehyde) fixed paraffin embedded mouse tissues. The
> > morphology of the section will be suboptimal due to this novel fixative.
> > If anybody has any experince with this Zinc Fixative please would
> > you
> share?
> >
> > Regards,
> >
> > Mesruh Turkekul
> > mskcc.org
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
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--
Mehmet Fatih BOZKURT, DVM, PhD
Afyon Kocatepe University
Faculty of Veterinary Medicine
Department of Pathology
03030, ANS Campus
Afyonkarahisar-TURKEY
Tel: +902722281312-16173/16237
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