[Histonet] ZInc formalin

[Mehmet Fatih BOZKURT]

Santa Cruz, SC-1506 is a good goat polyclonal antibody for mouse tissues.
also there is rabbit IgG that is coded SC-1506R.


On Thu, Sep 19, 2013 at 4:32 AM, Mesruh Turkekul <turkekul <@t> gmail.com> wrote:

> Hi Liz,
>
> I checked the web site and the images of FFPE sections stained with CD31
> from Dianova. It seems that it mostly stains large blood vessels. Which I
> have experienced with most CD31 Ab on the market. Does this ab stain all
> the capillaries in your experiments?
>
> Thanks again,
> Mesruh
>
> Sent from my iPad
>
> On Sep 18, 2013, at 7:51 PM, Elizabeth Chlipala <liz <@t> premierlab.com>
> wrote:
>
> > Dianova has an excellent antibody for CD31 in mice for FFPE tissue
> samples.
> >
> > Liz
> >
> > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> > Premier Laboratory, LLC
> > PO Box 18592
> > Boulder, CO 80308
> > (303) 682-3949 office
> > (303) 881-0763 cell
> > (303) 682-9060 fax
> > liz <@t> premierlab.com
> >
> > Ship to address:
> >
> > Premier Laboratory, LLC
> > 1567 Skyway Drive, Unit E
> > Longmont, CO 80504
> > ________________________________________
> > From: histonet-bounces <@t> lists.utsouthwestern.edu [
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mesru T [
> turkekul <@t> gmail.com]
> > Sent: Wednesday, September 18, 2013 4:29 PM
> > To: histonet <@t> lists.utsouthwestern.edu
> > Subject: [Histonet] ZInc formalin
> >
> > Dear Histonetters,
> >
> > I do not think there is a good CD31 (PECAM-1) antibody that will work on
> > FFPE mouse tissues. If you have one please let me know. We use CD34 rat
> > mono (Abcam) and Meca-32 Rat mono (Dev Hybridoma Bank Iowa Univ.) to
> label
> > endothelial cells of blood vessels in FFPE mouse tissues.
> > There is a CD31 Rat mono from BD that will work of 10% NBF fixed, sucrose
> > protected cryo embedded mouse tissues with antigen retrieval.
> > The same CD31 Rat mono from BD will also work on Zinc Fixative  from BD
> > cat# 51-7538KZ ( it is a solution of ZnCl2 and maybe other zinc salts but
> > no aldehyde) fixed paraffin embedded mouse tissues. The morphology of the
> > section will be suboptimal due to this novel fixative.
> > If anybody has any experince with this Zinc Fixative please would you
> share?
> >
> > Regards,
> >
> > Mesruh Turkekul
> > mskcc.org
> > _______________________________________________
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>
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-- 
Mehmet Fatih BOZKURT, DVM, PhD
Afyon Kocatepe University
Faculty of Veterinary Medicine
Department of Pathology
03030, ANS Campus
Afyonkarahisar-TURKEY
Tel: +902722281312-16173/16237