[Histonet] ZInc formalin

[BILL O'CONNOR]

Santa Cruz had the only really good antibody for CD31 in mouse that worked on FFPE.  Yes, Liz - their goat died.  I remember them telling me that about 10 years ago.  I was working on a project at the time where CD31 was so very important in murine xenografts.  No one has every really been able to get another antibody that was as good as that one.  They tried to clone the goat, but no mas.
Jackie



-----Original Message-----
From: Elizabeth Chlipala <liz <@t> premierlab.com>
To: Mehmet Fatih BOZKURT <fbozkurt <@t> gmail.com>; Mesruh Turkekul <turkekul <@t> gmail.com>; Histonet <Histonet <@t> lists.utsouthwestern.edu>
Sent: Thu, Sep 19, 2013 9:48 am
Subject: RE: [Histonet] ZInc formalin


Hello All
Unless something has changed,  this is how I remember it.   That antibody used 
o work for mouse on FFPE tissue samples ( I had a lot that did work and it was 
reat, but that was years ago) and then the goat died or something like 
hat??????.   From what I understand now the current lot numbers do not work on 
FPE tissues samples.  Has something changed recently that this antibody will 
ork again in FFPE mouse tissues, if so I'm interested.  That antibody worked on 
ultiple species quite well before.
Thanks
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
aboratory Manager
remier Laboratory, LLC
O Box 18592
oulder, CO 80308
ork (303) 682-3949
ax (303) 682-9060
ell (303) 881-0763
iz <@t> premierlab.com
ww.premierlab.com
Ship to address:
1567 Skyway Drive, Unit E
ongmont, CO 80504

-----Original Message-----
rom: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] 
n Behalf Of Mehmet Fatih BOZKURT
ent: Wednesday, September 18, 2013 10:41 PM
o: Mesruh Turkekul; Histonet <@t> lists.utsouthwestern.edu
ubject: Re: [Histonet] ZInc formalin
Santa Cruz, SC-1506 is a good goat polyclonal antibody for mouse tissues.
lso there is rabbit IgG that is coded SC-1506R.

n Thu, Sep 19, 2013 at 4:32 AM, Mesruh Turkekul <turkekul <@t> gmail.com> wrote:
> Hi Liz,

 I checked the web site and the images of FFPE sections stained with
 CD31 from Dianova. It seems that it mostly stains large blood vessels.
 Which I have experienced with most CD31 Ab on the market. Does this ab
 stain all the capillaries in your experiments?

 Thanks again,
 Mesruh

 Sent from my iPad

 On Sep 18, 2013, at 7:51 PM, Elizabeth Chlipala <liz <@t> premierlab.com>
 wrote:

 > Dianova has an excellent antibody for CD31 in mice for FFPE tissue
 samples.
 >
 > Liz
 >
 > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO
 > Box 18592 Boulder, CO 80308
 > (303) 682-3949 office
 > (303) 881-0763 cell
 > (303) 682-9060 fax
 > liz <@t> premierlab.com
 >
 > Ship to address:
 >
 > Premier Laboratory, LLC
 > 1567 Skyway Drive, Unit E
 > Longmont, CO 80504
 > ________________________________________
 > From: histonet-bounces <@t> lists.utsouthwestern.edu [
 histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mesru T [
 turkekul <@t> gmail.com]
 > Sent: Wednesday, September 18, 2013 4:29 PM
 > To: histonet <@t> lists.utsouthwestern.edu
 > Subject: [Histonet] ZInc formalin
 >
 > Dear Histonetters,
 >
 > I do not think there is a good CD31 (PECAM-1) antibody that will
 > work on FFPE mouse tissues. If you have one please let me know. We
 > use CD34 rat mono (Abcam) and Meca-32 Rat mono (Dev Hybridoma Bank
 > Iowa Univ.) to
 label
 > endothelial cells of blood vessels in FFPE mouse tissues.
 > There is a CD31 Rat mono from BD that will work of 10% NBF fixed,
 > sucrose protected cryo embedded mouse tissues with antigen retrieval.
 > The same CD31 Rat mono from BD will also work on Zinc Fixative  from
 > BD cat# 51-7538KZ ( it is a solution of ZnCl2 and maybe other zinc
 > salts but no aldehyde) fixed paraffin embedded mouse tissues. The
 > morphology of the section will be suboptimal due to this novel fixative.
 > If anybody has any experince with this Zinc Fixative please would
 > you
 share?
 >
 > Regards,
 >
 > Mesruh Turkekul
 > mskcc.org
 > _______________________________________________
 > Histonet mailing list
 > Histonet <@t> lists.utsouthwestern.edu
 > http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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--
ehmet Fatih BOZKURT, DVM, PhD
fyon Kocatepe University
aculty of Veterinary Medicine
epartment of Pathology
3030, ANS Campus
fyonkarahisar-TURKEY
el: +902722281312-16173/16237
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