[Histonet] ZInc formalin

[Mesruh Turkekul]

Hi Liz,

I checked the web site and the images of FFPE sections stained with CD31 from Dianova. It seems that it mostly stains large blood vessels. Which I have experienced with most CD31 Ab on the market. Does this ab stain all the capillaries in your experiments?

Thanks again,
Mesruh

Sent from my iPad

On Sep 18, 2013, at 7:51 PM, Elizabeth Chlipala <liz <@t> premierlab.com> wrote:

> Dianova has an excellent antibody for CD31 in mice for FFPE tissue samples.
> 
> Liz
> 
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Premier Laboratory, LLC
> PO Box 18592
> Boulder, CO 80308
> (303) 682-3949 office
> (303) 881-0763 cell
> (303) 682-9060 fax
> liz <@t> premierlab.com
> 
> Ship to address:
> 
> Premier Laboratory, LLC
> 1567 Skyway Drive, Unit E
> Longmont, CO 80504
> ________________________________________
> From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mesru T [turkekul <@t> gmail.com]
> Sent: Wednesday, September 18, 2013 4:29 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] ZInc formalin
> 
> Dear Histonetters,
> 
> I do not think there is a good CD31 (PECAM-1) antibody that will work on
> FFPE mouse tissues. If you have one please let me know. We use CD34 rat
> mono (Abcam) and Meca-32 Rat mono (Dev Hybridoma Bank Iowa Univ.) to label
> endothelial cells of blood vessels in FFPE mouse tissues.
> There is a CD31 Rat mono from BD that will work of 10% NBF fixed, sucrose
> protected cryo embedded mouse tissues with antigen retrieval.
> The same CD31 Rat mono from BD will also work on Zinc Fixative  from BD
> cat# 51-7538KZ ( it is a solution of ZnCl2 and maybe other zinc salts but
> no aldehyde) fixed paraffin embedded mouse tissues. The morphology of the
> section will be suboptimal due to this novel fixative.
> If anybody has any experince with this Zinc Fixative please would you share?
> 
> Regards,
> 
> Mesruh Turkekul
> mskcc.org
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