[Histonet] FISH enumeration

[Sue Hunter]

My techs do the scoring for the FISH testing.  We have a Leica Ariol system - the HER2 FISH are computer assisted, but the Urovysions are manually assessed.  That may change with the new systems we are receiving.  My techs are exceptionally well trained in morphology - they sit with our director to learn, and are really really good. We do about 10 urovysions per week and sometimes as many as 30 pathvysions per week.  I have one or two techs on the FISH rotation each week.  The other techs step in as needed.  The pathologist circles the area on the HER2 IHC slide to be FISHed.  The Pathologists do come down to  the lab to see cases, but they rely on the techs.  The pathologists also look at all other criteria before signing out the case to make sure everything fits. We are also getting a new image hub so the saved images will be viewable by the pathologists - this will be a really nice addition to the reviewing process. I believe there are many laboratories where the cytotechs read out the urovsyions.
Sue

Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shunter <@t> beaumont.edu


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of joelle weaver
Sent: Saturday, September 07, 2013 10:22 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] FISH enumeration

 For any laboratories out there who perform in house FISH procedures, if you could share what personnel are responsible for doing the signal enumeration & scoring? It would be helpful if you could describe the personnel's training and certification,  as well as an approximation of FTE's needed with some volumes. Do you do manual enumeration or use scanning software? 
Thanks for any input.





Joelle Weaver MAOM, HTL (ASCP) QIHC
 
> From: TMcNemar <@t> lmhealth.org
> To: thisisann <@t> aol.com; histonet <@t> lists.utsouthwestern.edu
> Date: Fri, 6 Sep 2013 05:45:41 -0400
> Subject: RE: [Histonet] Cell Block Preparation
> CC: 
> 
> This is how we do it now.  In the old days, we used agar and to my mind, it is still the best way when you have scant material.
> - Spin in a conical tube and pour off
> - Melt an agar slant (we get TSA slant from micro)
> - Pour the agar into the conical tube and spin for 5 minutes
> - The agar will re-solidify and whatever sediment there is will be concentrated in the very tip of the cone
> - The agar will slide out of the centrifuge tube
> - Slice off the very tip and wrap in lens paper
> - Place the wrapped tip in a cassette and process as usual
> - Embed the specimen tip down and you are good to go...
> 
> I still use this method today when I feel it necessary.  Works great.
> 
> Tom McNemar, HT(ASCP)
> Histology Co-ordinator
> Licking Memorial Health Systems
> (740) 348-4163
> (740) 348-4166
> tmcnemar <@t> lmhealth.org
> www.LMHealth.org
> 
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ann Specian
> Sent: Thursday, September 05, 2013 12:45 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Cell Block Preparation
> 
> 
> I am getting complaints in regard to "insufficient" cell blocks.  We currently spin, pour off the supernatant, retrieve the sediment and process in lens paper.
> 
> Does anyone have a more current technique which renders better cellularity?
> 
> Also, do you know which renders a better cell block:  a fresh specimen, a specimen fixed in Cytolyt or a specimen fixed in 10% NBF?
> 
> Thanks,
> Ann
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