[Histonet] Cell Block Preparation

[Dessoye, Michael J]

We have had mixed results in the past with cell blocks, but currently we
are filtering the fluid through a biopsy bag and processing that.  We
seem to be retaining more specimen with that method (we have used
sponges and lens paper in the past).

Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General
Hospital | An Affiliate of Commonwealth Health |
mjdessoye <@t> commonwealthhealth.net | 575 N. River Street | Wilkes Barre,
PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 
 

-----Original Message-----
From: Ann Specian [mailto:thisisann <@t> aol.com] 
Sent: Thursday, September 05, 2013 12:45 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Cell Block Preparation


I am getting complaints in regard to "insufficient" cell blocks.  We
currently spin, pour off the supernatant, retrieve the sediment and
process in lens paper.

Does anyone have a more current technique which renders better
cellularity?

Also, do you know which renders a better cell block:  a fresh specimen,
a specimen fixed in Cytolyt or a specimen fixed in 10% NBF?

Thanks,
Ann

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