AW: [Histonet] FISH enumeration

[Gudrun Lang]

Pathologists do FISH reading. Learning by doing. Manual enumeration.

Each doctor, who orders a FISH has to read it by him/herself. Often they do
it with a second person/second look for reliable results.

Gudrun Lang
Histolab,  Linz, Austria


-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von joelle
weaver
Gesendet: Samstag, 07. September 2013 16:22
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] FISH enumeration

 For any laboratories out there who perform in house FISH procedures, if you
could share what personnel are responsible for doing the signal enumeration
& scoring? It would be helpful if you could describe the personnel's
training and certification,  as well as an approximation of FTE's needed
with some volumes. Do you do manual enumeration or use scanning software? 
Thanks for any input.





Joelle Weaver MAOM, HTL (ASCP) QIHC
 
> From: TMcNemar <@t> lmhealth.org
> To: thisisann <@t> aol.com; histonet <@t> lists.utsouthwestern.edu
> Date: Fri, 6 Sep 2013 05:45:41 -0400
> Subject: RE: [Histonet] Cell Block Preparation
> CC: 
> 
> This is how we do it now.  In the old days, we used agar and to my mind,
it is still the best way when you have scant material.
> - Spin in a conical tube and pour off
> - Melt an agar slant (we get TSA slant from micro)
> - Pour the agar into the conical tube and spin for 5 minutes
> - The agar will re-solidify and whatever sediment there is will be
concentrated in the very tip of the cone
> - The agar will slide out of the centrifuge tube
> - Slice off the very tip and wrap in lens paper
> - Place the wrapped tip in a cassette and process as usual
> - Embed the specimen tip down and you are good to go...
> 
> I still use this method today when I feel it necessary.  Works great.
> 
> Tom McNemar, HT(ASCP)
> Histology Co-ordinator
> Licking Memorial Health Systems
> (740) 348-4163
> (740) 348-4166
> tmcnemar <@t> lmhealth.org
> www.LMHealth.org
> 
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ann Specian
> Sent: Thursday, September 05, 2013 12:45 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Cell Block Preparation
> 
> 
> I am getting complaints in regard to "insufficient" cell blocks.  We
currently spin, pour off the supernatant, retrieve the sediment and process
in lens paper.
> 
> Does anyone have a more current technique which renders better
cellularity?
> 
> Also, do you know which renders a better cell block:  a fresh specimen, a
specimen fixed in Cytolyt or a specimen fixed in 10% NBF?
> 
> Thanks,
> Ann
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