[Histonet] FISH enumeration

[joelle weaver]

 For any laboratories out there who perform in house FISH procedures, if you could share what personnel are responsible for doing the signal enumeration & scoring? It would be helpful if you could describe the personnel's training and certification,  as well as an approximation of FTE's needed with some volumes. Do you do manual enumeration or use scanning software? 
Thanks for any input.





Joelle Weaver MAOM, HTL (ASCP) QIHC
 
> From: TMcNemar <@t> lmhealth.org
> To: thisisann <@t> aol.com; histonet <@t> lists.utsouthwestern.edu
> Date: Fri, 6 Sep 2013 05:45:41 -0400
> Subject: RE: [Histonet] Cell Block Preparation
> CC: 
> 
> This is how we do it now.  In the old days, we used agar and to my mind, it is still the best way when you have scant material.
> - Spin in a conical tube and pour off
> - Melt an agar slant (we get TSA slant from micro)
> - Pour the agar into the conical tube and spin for 5 minutes
> - The agar will re-solidify and whatever sediment there is will be concentrated in the very tip of the cone
> - The agar will slide out of the centrifuge tube
> - Slice off the very tip and wrap in lens paper
> - Place the wrapped tip in a cassette and process as usual
> - Embed the specimen tip down and you are good to go...
> 
> I still use this method today when I feel it necessary.  Works great.
> 
> Tom McNemar, HT(ASCP)
> Histology Co-ordinator
> Licking Memorial Health Systems
> (740) 348-4163
> (740) 348-4166
> tmcnemar <@t> lmhealth.org
> www.LMHealth.org
> 
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ann Specian
> Sent: Thursday, September 05, 2013 12:45 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Cell Block Preparation
> 
> 
> I am getting complaints in regard to "insufficient" cell blocks.  We currently spin, pour off the supernatant, retrieve the sediment and process in lens paper.
> 
> Does anyone have a more current technique which renders better cellularity?
> 
> Also, do you know which renders a better cell block:  a fresh specimen, a specimen fixed in Cytolyt or a specimen fixed in 10% NBF?
> 
> Thanks,
> Ann
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you.
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet