[Histonet] Cell Block Preparation

[Daniel Hewitt]

We recently switched most of our cell blocks from agar to Histogel,
works great. We use small disposable embedding mold, put the specimen in
the bottom and add the histogel about half way up the mold, let it
harden, pop it out and put it in the cass. Also cuts much better than
agar.

Daniel Hewitt
Histology Supervisor, HVS
412-749-7371

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Rathborne, Toni
Sent: Friday, September 06, 2013 7:18 AM
To: 'Tom McNemar'; 'Ann Specian'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Cell Block Preparation

I wonder if this method could be used with the product Histogel. Has
anyone tried it?

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tom
McNemar
Sent: Friday, September 06, 2013 5:46 AM
To: 'Ann Specian'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Cell Block Preparation

This is how we do it now.  In the old days, we used agar and to my mind,
it is still the best way when you have scant material.
- Spin in a conical tube and pour off
- Melt an agar slant (we get TSA slant from micro)
- Pour the agar into the conical tube and spin for 5 minutes
- The agar will re-solidify and whatever sediment there is will be
concentrated in the very tip of the cone
- The agar will slide out of the centrifuge tube
- Slice off the very tip and wrap in lens paper
- Place the wrapped tip in a cassette and process as usual
- Embed the specimen tip down and you are good to go...

I still use this method today when I feel it necessary.  Works great.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ann
Specian
Sent: Thursday, September 05, 2013 12:45 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Cell Block Preparation


I am getting complaints in regard to "insufficient" cell blocks.  We
currently spin, pour off the supernatant, retrieve the sediment and
process in lens paper.

Does anyone have a more current technique which renders better
cellularity?

Also, do you know which renders a better cell block:  a fresh specimen,
a specimen fixed in Cytolyt or a specimen fixed in 10% NBF?

Thanks,
Ann
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