[Histonet] Cell Block Preparation

[Tony Auge]

We spin the sample down, pour off supernatant. Resuspend in 5 drops
thromboplastin and 4 drops human serum we receive from our phlebotomy lab.
Let firm for 5 minutes and then fix in NBF. The agar suggestion probably
works similarly if you can't get serum.
On Sep 6, 2013 9:39 AM, "Dessoye, Michael J" <
mjdessoye <@t> commonwealthhealth.net> wrote:

> We have had mixed results in the past with cell blocks, but currently we
> are filtering the fluid through a biopsy bag and processing that.  We
> seem to be retaining more specimen with that method (we have used
> sponges and lens paper in the past).
>
> Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General
> Hospital | An Affiliate of Commonwealth Health |
> mjdessoye <@t> commonwealthhealth.net | 575 N. River Street | Wilkes Barre,
> PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526
>
>
> -----Original Message-----
> From: Ann Specian [mailto:thisisann <@t> aol.com]
> Sent: Thursday, September 05, 2013 12:45 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Cell Block Preparation
>
>
> I am getting complaints in regard to "insufficient" cell blocks.  We
> currently spin, pour off the supernatant, retrieve the sediment and
> process in lens paper.
>
> Does anyone have a more current technique which renders better
> cellularity?
>
> Also, do you know which renders a better cell block:  a fresh specimen,
> a specimen fixed in Cytolyt or a specimen fixed in 10% NBF?
>
> Thanks,
> Ann
>
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