[Histonet] Cell Block Preparation

[Cartun, Richard]

We borrowed a wonderful cell block procedure from Windham Community Memorial Hospital here in CT several years ago.  The specimen is collected fresh in sterile saline or RPMI and then centrifuged to concentrate the cells.  Plasma and then thrombin are added to form a clot.  The clot is then fixed in formalin and processed in our Histology Laboratory.  I will send the procedure to anyone who is interested.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax
richard.cartun <@t> hhchealth.org

________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Ann Specian [thisisann <@t> aol.com]
Sent: Thursday, September 05, 2013 12:45 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Cell Block Preparation

I am getting complaints in regard to "insufficient" cell blocks.  We currently spin, pour off the supernatant, retrieve the sediment and process in lens paper.

Does anyone have a more current technique which renders better cellularity?

Also, do you know which renders a better cell block:  a fresh specimen, a specimen fixed in Cytolyt or a specimen fixed in 10% NBF?

Thanks,
Ann
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