[Histonet] Re: Unencased Amoeba Stain

[Bob Richmond]

It's better not to assume that a stain developed for smears will work
on tissue sections without modification. The Gram stain is an obvious
case in point.

Bob Richmond
Samurai Pathologist
Maryville TN
*********************************************
On Wed, Feb 27, 2013 at 9:20 PM, Tony Reilly
<Tony_Reilly <@t> health.qld.gov.au> wrote:
> It is true that I have never used it on tissue sections as I have usually
> performed it on smears for the  Microbiology department.  However some of
> the specimens stained were formalin fixed and stained well while others were
> PVA fixed.
>
> regards
> Tony
>
>
>
>
> Tony Reilly  B.App.Sc. , M.Sc.
>
> Chief Scientist, Anatomical Pathology
>
> Pathology Queensland-PA Laboratory
>
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>>>> Bob Richmond <rsrichmond <@t> gmail.com> 2/28/2013 12:12 pm >>>
>
> You're referring I suppose to the "Gomori-Wheatley" modification of
> the one step trichrome, used for staining intestinal amoebae. Don't
> know if it will work on tissue sections. It has quite a number of
> variants.
>
> Once again, I'd advise consulting an eye pathologist.
>
> Bob Richmond
> Samurai Pathologist
> Maryville TN
> *****************
> On Wed, Feb 27, 2013 at 8:58 PM, Tony Reilly
> <Tony_Reilly <@t> health.qld.gov.au> wrote:
>> Hi Matt
>>
>> The Heidenhain's stain is good but if you want one that is prettier try
>> Gomori's one step trichrome.  I have only ever used it for intestinal
>> protozoa but I think it would work for all.
>>
>> regards
>> Tony
>>
>> Tony Reilly  B.App.Sc. , M.Sc.
>>
>> Chief Scientist, Anatomical Pathology
>>
>> Pathology Queensland-PA Laboratory
>>
>> ________________________________________________
>> Health Services Support Agency | Department of Health
>>
>> Level 1, Building 15,Princess Alexandra Hospital
>>
>> Ipswich Road,WOOLLOONGABBA  Qld 4102
>> Ph: 07 3176 2412
>> Mob: 0402 139411
>>
>> Fax: 07 3176 2930
>>
>> Email: tony_reilly <@t> health.qld.gov.au
>>
>> Web:  www.health.qld.gov.au/qhcss/
>>
>>>>> Bob Richmond <rsrichmond <@t> gmail.com> 2/28/2013 4:19 am >>>
>>
>> Matt Brooks asks: >>One of our Neuropath docs is inquiring about a
>> special stain for unencased
>> [amoebae] in cornea biopsies. I did a search and Gridley's method was
>> the best option that appreaded [sic]. Is there someone that could
>> either verify this stain will work on this organism or let me know
>> what you stain you are using?<< and René Buesa suggests Heidenhain's
>> iron hematoxylin.
>>
>> Corneal ulcers are caused by Acanthamoeba and related species, rather
>> than by Entamoeba histolytica. Google >>amoeba corneal stain<< - some
>> references suggest a fluorescent stain such as calcofluor white, if
>> you have access to a fluorescence microscope.
>>
>> Iron hematoxylins provide exquisite nuclear detail, but are
>> challenging to set up for the first time (I've done it), and are not
>> specific.
>>
>> Periodic acid Schiff (PAS) is often used to demonstrate the glycogen
>> in Entamoeba histolytica trophozoites, but I don't know if the other
>> pathogenic amoebae contain glycogen.
>>
>> My advice would be to ask one of the academic eye pathologists. My
>> choice would be Dr. Hans Grossniklaus at Emory, but there are a good
>> many more. And remember you'll need to find a control slide.
>>
>> Bob Richmond
>> Samurai Pathologist
>> Maryville TN
>
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