[Histonet] Re: Unencased Amoeba Stain

[Paula Pierce]

Greetings,

once infected tissue is placed in fixative, Acanthamoeba form a double walled encasement that stains nicely with any trichrome stain. Light green works better than aniline blue. The amoeba are large and measure approximately 30 microns.

There are about 100 human corneal cases a year in the U.S. and one year back in the 80s we saw 10 of those cases.

 
Paula K. Pierce, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
Specializing in Eye Histology

8901 S. Santa Fe, Suite G
Oklahoma City, OK 73139
405-759-3953 Lab
405-759-7513 Fax
www.excaliburpathology.com


________________________________
 From: Bob Richmond <rsrichmond <@t> gmail.com>
To: Tony Reilly <Tony_Reilly <@t> health.qld.gov.au> 
Cc: histonet <@t> lists.utsouthwestern.edu 
Sent: Wednesday, February 27, 2013 8:12 PM
Subject: Re: [Histonet] Re: Unencased Amoeba Stain
 
You're referring I suppose to the "Gomori-Wheatley" modification of
the one step trichrome, used for staining intestinal amoebae. Don't
know if it will work on tissue sections. It has quite a number of
variants.

Once again, I'd advise consulting an eye pathologist.

Bob Richmond
Samurai Pathologist
Maryville TN
*****************
On Wed, Feb 27, 2013 at 8:58 PM, Tony Reilly
<Tony_Reilly <@t> health.qld.gov.au> wrote:
> Hi Matt
>
> The Heidenhain's stain is good but if you want one that is prettier try
> Gomori's one step trichrome.  I have only ever used it for intestinal
> protozoa but I think it would work for all.
>
> regards
> Tony
>
> Tony Reilly  B.App.Sc. , M.Sc.
>
> Chief Scientist, Anatomical Pathology
>
> Pathology Queensland-PA Laboratory
>
> ________________________________________________
> Health Services Support Agency | Department of Health
>
> Level 1, Building 15,Princess Alexandra Hospital
>
> Ipswich Road,WOOLLOONGABBA  Qld 4102
> Ph: 07 3176 2412
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> Email: tony_reilly <@t> health.qld.gov.au
>
> Web:  www.health.qld.gov.au/qhcss/
>
>>>> Bob Richmond <rsrichmond <@t> gmail.com> 2/28/2013 4:19 am >>>
>
> Matt Brooks asks: >>One of our Neuropath docs is inquiring about a
> special stain for unencased
> [amoebae] in cornea biopsies. I did a search and Gridley's method was
> the best option that appreaded [sic]. Is there someone that could
> either verify this stain will work on this organism or let me know
> what you stain you are using?<< and René Buesa suggests Heidenhain's
> iron hematoxylin.
>
> Corneal ulcers are caused by Acanthamoeba and related species, rather
> than by Entamoeba histolytica. Google >>amoeba corneal stain<< - some
> references suggest a fluorescent stain such as calcofluor white, if
> you have access to a fluorescence microscope.
>
> Iron hematoxylins provide exquisite nuclear detail, but are
> challenging to set up for the first time (I've done it), and are not
> specific.
>
> Periodic acid Schiff (PAS) is often used to demonstrate the glycogen
> in Entamoeba histolytica trophozoites, but I don't know if the other
> pathogenic amoebae contain glycogen.
>
> My advice would be to ask one of the academic eye pathologists. My
> choice would be Dr. Hans Grossniklaus at Emory, but there are a good
> many more. And remember you'll need to find a control slide.
>
> Bob Richmond
> Samurai Pathologist
> Maryville TN

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