[Histonet] Re: Unencased Amoeba Stain

[Tony Reilly]

It is true that I have never used it on tissue sections as I have
usually performed it on smears for the  Microbiology department. 
However some of the specimens stained were formalin fixed and stained
well while others were PVA fixed.
 
regards
Tony
 
 
 

Tony Reilly  B.App.Sc. , M.Sc.
Chief Scientist, Anatomical Pathology
Pathology Queensland-PA Laboratory 
________________________________________________
Health Services Support Agency | Department of Health
 
Level 1, Building 15,Princess Alexandra Hospital
Ipswich Road,WOOLLOONGABBA  Qld4102
Ph: 07 3176 2412
Mob: 0402 139411
Fax: 07 3176 2930
Email: tony_reilly <@t> health.qld.gov.au
Web:  www.health.qld.gov.au/qhcss/
 
 


>>> Bob Richmond <rsrichmond <@t> gmail.com> 2/28/2013 12:12 pm >>>
You're referring I suppose to the "Gomori-Wheatley" modification of
the one step trichrome, used for staining intestinal amoebae. Don't
know if it will work on tissue sections. It has quite a number of
variants.

Once again, I'd advise consulting an eye pathologist.

Bob Richmond
Samurai Pathologist
Maryville TN
*****************
On Wed, Feb 27, 2013 at 8:58 PM, Tony Reilly
<Tony_Reilly <@t> health.qld.gov.au> wrote:
> Hi Matt
>
> The Heidenhain's stain is good but if you want one that is prettier
try
> Gomori's one step trichrome.  I have only ever used it for
intestinal
> protozoa but I think it would work for all.
>
> regards
> Tony
>
> Tony Reilly  B.App.Sc. , M.Sc.
>
> Chief Scientist, Anatomical Pathology
>
> Pathology Queensland-PA Laboratory
>
> ________________________________________________
> Health Services Support Agency | Department of Health
>
> Level 1, Building 15,Princess Alexandra Hospital
>
> Ipswich Road,WOOLLOONGABBA  Qld 4102
> Ph: 07 3176 2412
> Mob: 0402 139411
>
> Fax: 07 3176 2930
>
> Email: tony_reilly <@t> health.qld.gov.au 
>
> Web:  www.health.qld.gov.au/qhcss/ 
>
>>>> Bob Richmond <rsrichmond <@t> gmail.com> 2/28/2013 4:19 am >>>
>
> Matt Brooks asks: >>One of our Neuropath docs is inquiring about a
> special stain for unencased
> [amoebae] in cornea biopsies. I did a search and Gridley's method
was
> the best option that appreaded [sic]. Is there someone that could
> either verify this stain will work on this organism or let me know
> what you stain you are using?<< and René Buesa suggests Heidenhain's
> iron hematoxylin.
>
> Corneal ulcers are caused by Acanthamoeba and related species,
rather
> than by Entamoeba histolytica. Google >>amoeba corneal stain<< -
some
> references suggest a fluorescent stain such as calcofluor white, if
> you have access to a fluorescence microscope.
>
> Iron hematoxylins provide exquisite nuclear detail, but are
> challenging to set up for the first time (I've done it), and are not
> specific.
>
> Periodic acid Schiff (PAS) is often used to demonstrate the glycogen
> in Entamoeba histolytica trophozoites, but I don't know if the other
> pathogenic amoebae contain glycogen.
>
> My advice would be to ask one of the academic eye pathologists. My
> choice would be Dr. Hans Grossniklaus at Emory, but there are a good
> many more. And remember you'll need to find a control slide.
>
> Bob Richmond
> Samurai Pathologist
> Maryville TN

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