[Histonet] Re: Unencased Amoeba Stain

[Bob Richmond]

You're referring I suppose to the "Gomori-Wheatley" modification of
the one step trichrome, used for staining intestinal amoebae. Don't
know if it will work on tissue sections. It has quite a number of
variants.

Once again, I'd advise consulting an eye pathologist.

Bob Richmond
Samurai Pathologist
Maryville TN
*****************
On Wed, Feb 27, 2013 at 8:58 PM, Tony Reilly
<Tony_Reilly <@t> health.qld.gov.au> wrote:
> Hi Matt
>
> The Heidenhain's stain is good but if you want one that is prettier try
> Gomori's one step trichrome.  I have only ever used it for intestinal
> protozoa but I think it would work for all.
>
> regards
> Tony
>
> Tony Reilly  B.App.Sc. , M.Sc.
>
> Chief Scientist, Anatomical Pathology
>
> Pathology Queensland-PA Laboratory
>
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>
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>>>> Bob Richmond <rsrichmond <@t> gmail.com> 2/28/2013 4:19 am >>>
>
> Matt Brooks asks: >>One of our Neuropath docs is inquiring about a
> special stain for unencased
> [amoebae] in cornea biopsies. I did a search and Gridley's method was
> the best option that appreaded [sic]. Is there someone that could
> either verify this stain will work on this organism or let me know
> what you stain you are using?<< and René Buesa suggests Heidenhain's
> iron hematoxylin.
>
> Corneal ulcers are caused by Acanthamoeba and related species, rather
> than by Entamoeba histolytica. Google >>amoeba corneal stain<< - some
> references suggest a fluorescent stain such as calcofluor white, if
> you have access to a fluorescence microscope.
>
> Iron hematoxylins provide exquisite nuclear detail, but are
> challenging to set up for the first time (I've done it), and are not
> specific.
>
> Periodic acid Schiff (PAS) is often used to demonstrate the glycogen
> in Entamoeba histolytica trophozoites, but I don't know if the other
> pathogenic amoebae contain glycogen.
>
> My advice would be to ask one of the academic eye pathologists. My
> choice would be Dr. Hans Grossniklaus at Emory, but there are a good
> many more. And remember you'll need to find a control slide.
>
> Bob Richmond
> Samurai Pathologist
> Maryville TN