[Histonet] Re: zebrafish and IHC

[Hobbs, Carl]

Good point by Teri Johnson.
I don't decalcify, thus my results may well be compromised by this procedure....and, sure, the bone is disrupted.
I am only interested in CNS/PNS so, I don't worry about bone/cartilage disruption.
I fix in std 10% NBF.
Imho, it is better to "overfix" rather than to worry about optimal short fixation, if you are using HIER.

Utilitarily,

Carl

Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
School of Biomedical Sciences
Kings College London
Guys Campus
SE1 1UL
Tel: 020 78486813
Fax: 020 78486816
020 78486813