[Histonet] Luxol Fast Blue +PAS staining on rat spinal cord

[abijag]

Dear Fello histonetters,

I am looking for demonstration of demyelination in rat spinal cord paraffin sections. A quick look into the literature revealed use of combination of luxol fast blue and PAS for demonstration of demyelination and myelin breakdown products. Anybody over here will suggest me the procedure for doing this staining. There is also kit from hitobiotec for this staining. Now my questions are

1. Whats your preferred stain for demonstrating demyleination?

2. Any experience of using LFB+PAS for demonstration of the same

3. Any idea about the performance of this staining kit from hitobiotec



Thanks in advance