[Histonet] sections falling off

[tahseen <@t> brain.net.pk]

Dear Megha Kumar,
Use commercial + charged OR silanized slides.

Silanized slides are prepared by cleaning the slides by washing, followed
with a rinse in 95% ethanol. 4ml of 3-aminopropyltriethoxy silane is added
to 200 ml of acetone and slides are dipped for 30-60 seconds, followed by
60 seconds in agitated distilled water. The coated slides are then dried
for 1 hour, and can be boxed for future use.
References:Bancroft sixth edition page 694

Regards
Muhammad Tahseen
Senior Supervisor
Histopathology
SKMCH&RC


> Hi Histonetters !
> Can anyone suggest how to prevent  adult mouse skin sections from falling
> off during in situ hybridization? How to make sections adhere better to
> slides? I am using poly-lysine coated slides for 5-10 micron thick skin
> sections. The tissue is fixed in 4% PFA and embedded in paraffin.
> Please help !
> thanks
> megha
>
>
> --
> Megha Kumar, Ph.D.
> Post-doctoral Research Scholar
> Sen-Bandyopadhyay Lab
> Department of Biological Sciences and Bioengineering
> Indian Institute of Technology Kanpur
> Kanpur, 208016
> India
>
> phone - 9695830033
>
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