[Histonet] Antibody IDH1

[Bitting, Angela K.]

I received a spec sheet from Dianova that has their Ventana XT protocol. It suggests using CC2 standard retrieval and incubation for 32 minutes with amp. They give a dilution range of 1:20-1:50. It seems to work well for us although we are still validating. I hope that helps.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Roger Heyna
Sent: Friday, February 22, 2013 4:52 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Antibody IDH1

Billie,
 
We use Dianova's antibody and purchase it from Fisher. We've performed this stain for a little over six months and seem to be having success with the following protocol:
 
Dianova's IDH1 R132H Antibody, Diluted 1:100 Ventana Benchmark XT, iView Detection Standard CC1
37 degrees C
32 minute antibody incubation
A/B Block
 
I hope this helps!
 
Roger Heyna
Maywood, IL

>>> "Zimmerman, Billie" <BZIMMERM <@t> gru.edu> 2/22/2013 3:20 PM >>>
Does anyone perform this antibody?? We can't get it to work on the Ventana Ultra.  PhenoPath in Seattle performs it on the Dako autostainer using a small size polymer detection kit.  I would appreciate any input.

Thanks,

Billie Zimmerman


Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM <@t> gru.edu. Please update your address book to reflect this change.
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