[Histonet] Antibody IDH1
Richard Cartun
Rcartun <@t> harthosp.org
Fri Feb 22 15:59:31 CST 2013
We run it on Leica's Bond Max with good results. We use the mouse mAb (Clone H09) from "dianova" at a dilution of 1:50 (15' incubation) following low-pH retrieval.
Richard
Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596
(860) 545-2204 Fax
>>> "Zimmerman, Billie" <BZIMMERM <@t> gru.edu> 2/22/2013 4:20 PM >>>
Does anyone perform this antibody?? We can't get it to work on the Ventana Ultra. PhenoPath in Seattle performs it on the Dako autostainer using a small size polymer detection kit. I would appreciate any input.
Thanks,
Billie Zimmerman
Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM <@t> gru.edu. Please update your address book to reflect this change.
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