[Histonet] RE: Macrophage stain

[木村裕樹]

Hi. all
Let me add another question linked to marcophage staining.
So far we have done macrophage staining several times with F4/80, and
in most of the cases
IHC images were quite beautiful, for liver, spleen, adipose tissue and so on.
BUT, we have not succeeded in staining lung alveolar macrophages....
One of my friends(pathologist) told me that F4/80 was not good for
alveolar macrophage
but he also don't know how to deal with this problem.
Does anyone know what is appropriate antibody for staining lung
alveolar macrophage?
Thanks in advance

Hiro




2012/6/20 Elizabeth Chlipala <liz <@t> premierlab.com>:
> Serotec has two antibodies that will work a F4/80 and CD68, both are rat anti-mouse.  We have used them in the same application that you will be using (mouse aorta) with good success.
>
> Liz
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Premier Laboratory, LLC
> PO Box 18592
> Boulder, CO 80308
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> liz <@t> premierlab.com
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> ________________________________________
> From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Daniela Bodemer [daniela.bodemer <@t> mcri.edu.au]
> Sent: Tuesday, June 19, 2012 7:30 PM
> To: Histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Macrophage stain
>
> Hi all,
>
>
>
> I would like to stain for macrophages in mice aorta (5um frozen
> sections). Would you have suggestions on which stains to use and perhaps
> share the protocol?
>
>
>
> Thanks in advance,
>
>
>
> Daniela Bodemer
>
> Research Assistant
>
> Surgical Research, Infection and Immunity
>
>
>
> Murdoch Childrens Research Institute
>
> The Royal Children's Hospital
>
> Flemington Road Parkville Victoria 3052 Australia
>
> T 03 9936 6676     T (03 9936 6794)
>
> E daniela.bodemer <@t> mcri.edu.au <mailto:firstname.surname <@t> mcri.edu.au>
>
> www.mcri.edu.au <http://www.mcri.edu.au/>
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>
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