[Histonet] RE: Macrophage stain

[Peter Tree]

Hiro,
You are correct, alveolar macrophages do express less F4/80 than other tissue macrophages. I refer you to, for example:
Zhang X, Goncalves R, Mosser DM. The isolation and characterization of murine
macrophages. Curr Protoc Immunol. 2008 Nov;Chapter 14:Unit 14.1. PubMed PMID:
19016445; PubMed Central PMCID: PMC2834554.
Here they state:
Alveolar macrophages are functionally different from peritoneal macrophages. Peritoneal macrophages are F4/80high, CD11bhigh, and CD68+. Bone marrow-derived macrophages also express F4/80high, CD11bhigh, and CD68+. However, alveolar macrophages display a F4/80low, CD11blow, and CD68+ phenotype under normal physiological conditions.

Staining alveolar macrophages with CD68 (clone FA-11) would be a practical alternative for cryostat material, it just looks less pretty. Good luck.
Peter Tree
Technical Data Manager
AbD Serotec
Morphosys UK Ltd
Endeavour house
Langford Lane
Kidlington
Oxon
OX5 1GE

e.mail:  peter.tree <@t> abdserotec.com




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