[Histonet] hrp vs alk phos

[Heather Mcleod]

Dear Histonetters,
Our pathologist wants us to change from HRP to ALK PHOS.  We run a Ventana XT
using the titration option.  Fifteen of our 20 antibody selection are giving
the same result when run concurrently with the 2 detection systems. The other
5 stain perfectly with the HRP kit and gives NO staining with the ALK PHOS
kit.  We use the same protocol except for the kit selection and the titrated
antibody is taken from the same vial for the respective pairs.  Has anybody
had a problem like this?  The answer may be obvious but we cannot see it
..... yet.
Please help
Many many thanks
Heather


















On Thu, 2 Feb 2012 15:31:04 +0100 "Gudrun Lang" <gu.lang <@t> gmx.at> wrote

> We have been performing C4d IHC on FFPE kidney for a couple of years. We
> receive only fixed samples.
> Yesterday my doctors came with this idea of IF - I still have to ask for the
> special reasons. But I have the suspicion, that they are not aware of the
> fact, that IF on frozen unfixed tissue is the usual way found in literature.
> 
> There are some articles that deal with comparison of IF(frozen) and
> IHC(ffpe). The results are usually an equal outcome. IF(frozen) shows
> additional staining in glomeruli.
> 
> Perhaps someone told them, that IF is the golden standard and recommended.
> 
> Gudrun
> 
> -----Ursprüngliche Nachricht-----
> Von: Richard Cartun [mailto:Rcartun <@t> harthosp.org] 
> Gesendet: Mittwoch, 01. Februar 2012 21:21
> An: gu.lang <@t> gmx.at
> Betreff: Re: [Histonet] C4d IF on FFPE kidney
> 
> Hi Gudrun:
> 
> Is there reason why you want to use IF and not immunoperoxidase?
> 
> Richard
> 
> Richard W. Cartun, MS, PhD
> Director, Histology & Immunopathology
> Director, Biospecimen Collection Programs
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT  06102
> (860) 545-1596 Office
> (860) 545-2204 Fax
> 
> 
> >>> "Gudrun Lang" <gu.lang <@t> gmx.at> 2/1/2012 3:17 PM >>>
> Hi!
> 
> Can someone provide me a immunofluorescence protocol for C4d on formalin
> fixed human paraffin sections?
> 
>  
> 
> thanks in advance
> 
>  
> 
> Gudrun Lang
> 
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