AW: [Histonet] C4d IF on FFPE kidney

[Tony Reilly]

Hi Gudren
 
We have been doing both IF and IHC for C4d for quite a while.  While we
have found that the IF staining is superior to the IHC we are not
unhappy with the IHC results.  Our IHC results have improved since
getting a Ventana Ultra which has allowed better control of the
staining.
 
regards
Tony
 
 
 
 

Tony Reilly  B.App.Sc. , M.Sc.
Chief Scientist, Anatomical Pathology
Pathology Queensland-PA Laboratory
_________________________________________________
Clinical and Statewide Services Division| QueenslandHealth
 
Level 1, Building 15,Princess Alexandra Hospital
Ipswich Road,WOOLLOONGABBA  Qld4102
Ph: 07 3176 2412
Mob: 0402 139411
Fax: 07 3176 2930
Email: tony_reilly <@t> health.qld.gov.au
Web:  www.health.qld.gov.au/qhcss/
 
 


>>> "Gudrun Lang" <gu.lang <@t> gmx.at> 2/3/2012 12:31 am >>>
We have been performing C4d IHC on FFPE kidney for a couple of years.
We
receive only fixed samples.
Yesterday my doctors came with this idea of IF - I still have to ask
for the
special reasons. But I have the suspicion, that they are not aware of
the
fact, that IF on frozen unfixed tissue is the usual way found in
literature.

There are some articles that deal with comparison of IF(frozen) and
IHC(ffpe). The results are usually an equal outcome. IF(frozen) shows
additional staining in glomeruli.

Perhaps someone told them, that IF is the golden standard and
recommended.

Gudrun

-----Ursprüngliche Nachricht-----
Von: Richard Cartun [mailto:Rcartun <@t> harthosp.org] 
Gesendet: Mittwoch, 01. Februar 2012 21:21
An: gu.lang <@t> gmx.at 
Betreff: Re: [Histonet] C4d IF on FFPE kidney

Hi Gudrun:

Is there reason why you want to use IF and not immunoperoxidase?

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


>>> "Gudrun Lang" <gu.lang <@t> gmx.at> 2/1/2012 3:17 PM >>>
Hi!

Can someone provide me a immunofluorescence protocol for C4d on
formalin
fixed human paraffin sections?



thanks in advance



Gudrun Lang

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