AW: [Histonet] C4d IF on FFPE kidney

[Gudrun Lang]

We have been performing C4d IHC on FFPE kidney for a couple of years. We
receive only fixed samples.
Yesterday my doctors came with this idea of IF - I still have to ask for the
special reasons. But I have the suspicion, that they are not aware of the
fact, that IF on frozen unfixed tissue is the usual way found in literature.

There are some articles that deal with comparison of IF(frozen) and
IHC(ffpe). The results are usually an equal outcome. IF(frozen) shows
additional staining in glomeruli.

Perhaps someone told them, that IF is the golden standard and recommended.

Gudrun

-----Ursprüngliche Nachricht-----
Von: Richard Cartun [mailto:Rcartun <@t> harthosp.org] 
Gesendet: Mittwoch, 01. Februar 2012 21:21
An: gu.lang <@t> gmx.at
Betreff: Re: [Histonet] C4d IF on FFPE kidney

Hi Gudrun:

Is there reason why you want to use IF and not immunoperoxidase?

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


>>> "Gudrun Lang" <gu.lang <@t> gmx.at> 2/1/2012 3:17 PM >>>
Hi!

Can someone provide me a immunofluorescence protocol for C4d on formalin
fixed human paraffin sections?

 

thanks in advance

 

Gudrun Lang

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