[Histonet] Abs for detecting immune cells in mouse FFPE sections

[monettes <@t> mskcc.org]

These are two good databases for antibodies and protocols for murine IHC: 

From the pathology lab at The Jackson Lab: 
http://tumor.informatics.jax.org/html/antibodies.html

From the animal pathology lab at NCI:
http://web.ncifcrf.gov/rtp/lasp/phl/immuno/

Best, 
Sebastien

Sébastien Monette, DMV, MVSc, DACVP
Comparative Pathologist
Tri-Institutional Laboratory of Comparative Pathology
Center of Comparative Medicine and Pathology
Memorial Sloan-Kettering Cancer Center
Weill Cornell Medical College
The Rockefeller University

1275 York Avenue, Box 270, New York NY 10065
Phone: 646-888-2420
Fax: 646-422-0139
Email MSKCC: monettes <@t> mskcc.org
Email WCMC: sem2010 <@t> med.cornell.edu
Web site: http://www.mskcc.org/mskcc/html/92361.cfm


-----Original Message-----
From: Yu, Jian [mailto:YuJ2 <@t> upmc.edu] 
Sent: Wednesday, October 26, 2011 3:44 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Abs for detecting immune cells in mouse FFPE sections

Dear all,

It will be greatly appreciated if you can share experience/information on the Abs against immune cells (vendor and cat#) that work for IHC or IF using mouse paraffin sections.  Info on alternative Abs for these populations can be helpful too.  I have a small research lab with limited budget for buying new Abs.  

Gr-1 (granulocytes) 
B220 (B cells) 
CD3 (T cells) 

Thank you!
 
Jian Yu, Ph.D.
University of Pittsburgh



 
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