[Histonet] Abs for detecting immune cells in mouse FFPE sections

[Jan Shivers]

> CD3 - rabbit polyclonal Ab from ThermoScientific (cat. # RB-360) works on 
> mouse FFPE tissue.
>
> Jan Shivers
> Senior Scientist
> Histology/IHC/EM Section Head
> Pathology Teaching Program
> University of Minnesota
> Veterinary Diagnostic Laboratory
> 1333 Gortner Ave.
> St. Paul, MN  55108
> 612-624-7297
> shive003 <@t> umn.edu
>
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> ----- Original Message ----- 
> From: "Yu, Jian" <YuJ2 <@t> upmc.edu>
> To: <histonet <@t> lists.utsouthwestern.edu>
> Sent: Wednesday, October 26, 2011 2:44 PM
> Subject: [Histonet] Abs for detecting immune cells in mouse FFPE sections
>
>
> Dear all,
>
> It will be greatly appreciated if you can share experience/information on 
> the Abs against immune cells (vendor and cat#) that work for IHC or IF 
> using mouse paraffin sections.  Info on alternative Abs for these 
> populations can be helpful too.  I have a small research lab with limited 
> budget for buying new Abs.
>
> Gr-1 (granulocytes)
> B220 (B cells)
> CD3 (T cells)
>
> Thank you!
>
> Jian Yu, Ph.D.
> University of Pittsburgh
>
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