[Histonet] RE: Abs for detecting immune cells in mouse FFPE sections

[Anatoli Gleiberman]

CD3 Rabbit monoclonal from Fisher (MA1-39552) works fine in IF on mouse paraffin sections,
B220 rat monoclonal from eBioscience (14-0452-85) works fine in IF on mouse paraffin sections. Used both in 1:100 dilution.

Anatoli Gleiberman, PhD
Director of Histopathology
Cleveland Biolabs, Inc
73 High Street
Buffalo, NY 14203
phone:716-849-6810 ext.354
fax:716-849-6817
e-mail: AGleiberman <@t> cbiolabs.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Yu, Jian
Sent: Wednesday, October 26, 2011 3:44 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Abs for detecting immune cells in mouse FFPE sections 

Dear all,

It will be greatly appreciated if you can share experience/information on the Abs against immune cells (vendor and cat#) that work for IHC or IF using mouse paraffin sections.  Info on alternative Abs for these populations can be helpful too.  I have a small research lab with limited budget for buying new Abs.  

Gr-1 (granulocytes) 
B220 (B cells) 
CD3 (T cells) 

Thank you!
 
Jian Yu, Ph.D.
University of Pittsburgh

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