[Histonet] oops

[William]

Oops. Yes hematoxylin staining interferes with DAB staining. Sorry!  You can save the slides by re-retrieving and restaining. 

Will

Sent from my iPhone

On Oct 27, 2011, at 10:59 AM, Denise G Crowley <dencrowl <@t> MIT.EDU> wrote:

> Hi all,
> 
> Bad day here.  Left the IHC stainer to attend a meeting and the volume of the DAB was not sufficient to stain all slides.  By the time I realized this, they had been counterstained.  I immediately applied DAB to the affected slides but naturally the antigen is not abundant if present at all.  Will this work or does the hematoxylin counterstain interfere with the peroxidase reaction?
> 
> Denise Crowley
> Histology Facility Manager
> Koch Center for Integrative Cancer Research
> Massachusetts Institute of Technology
> 500 Main St. 76-182
> Cambridge MA 02139
> 617-258-8183
> dencrowl <@t> mit.edu
> 
> 
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