[Histonet] oops

[Denise G Crowley]

Hi all,

Bad day here.  Left the IHC stainer to attend a meeting and the volume of the DAB was not sufficient to stain all slides.  By the time I realized this, they had been counterstained.  I immediately applied DAB to the affected slides but naturally the antigen is not abundant if present at all.  Will this work or does the hematoxylin counterstain interfere with the peroxidase reaction?

Denise Crowley
Histology Facility Manager
Koch Center for Integrative Cancer Research
Massachusetts Institute of Technology
500 Main St. 76-182
Cambridge MA 02139
617-258-8183
dencrowl <@t> mit.edu