[Histonet] oops

[Patsy Ruegg]

I have done cs before DAB lots of times with no problems, the only time I
would expect a problem would be if your antigen is expressed in the nuclei
and the cs is too dark.

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of William
Sent: Thursday, October 27, 2011 12:03 PM
To: Denise G Crowley
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] oops

Oops. Yes hematoxylin staining interferes with DAB staining. Sorry!  You can
save the slides by re-retrieving and restaining. 

Will

Sent from my iPhone

On Oct 27, 2011, at 10:59 AM, Denise G Crowley <dencrowl <@t> MIT.EDU> wrote:

> Hi all,
> 
> Bad day here.  Left the IHC stainer to attend a meeting and the volume of
the DAB was not sufficient to stain all slides.  By the time I realized
this, they had been counterstained.  I immediately applied DAB to the
affected slides but naturally the antigen is not abundant if present at all.
Will this work or does the hematoxylin counterstain interfere with the
peroxidase reaction?
> 
> Denise Crowley
> Histology Facility Manager
> Koch Center for Integrative Cancer Research
> Massachusetts Institute of Technology
> 500 Main St. 76-182
> Cambridge MA 02139
> 617-258-8183
> dencrowl <@t> mit.edu
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet