[Histonet] Use of ammonia water

[Bartlett, Jeanine (CDC/OID/NCEZID)]

I  use it on particularly difficult blocks and have seen no ill effects.

Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590 
jeanine.bartlett <@t> cdc.hhs.gov


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Sharon.Davis-Devine
Sent: Monday, December 27, 2010 3:58 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Use of ammonia water

Happy Holidays Histonetters, I have a question for you. Do any of you
use ammonia water to soften the blocks before cutting? If so, does this
have any effect  on IHC staining? Any help will be greatly appreciated.
Thanks.

Sharon Davis-Devine, CT (ASCP)
Cytology-Histology  Supervisor
Carle Foundation Hospital
Laboratory and Pathology Services
611 West Park Street
Urbana, Illinois 61801
217-383-3572
sharon.davis-devine <@t> carle.com

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