[Histonet] RE: Use of ammonia water

[Susan.Walzer <@t> HCAHealthcare.com]

We have always used water with liquid soap and some ammonia to soften blocks. It has had no effect on IHC.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine
Sent: Monday, December 27, 2010 3:58 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Use of ammonia water

Happy Holidays Histonetters, I have a question for you. Do any of you use ammonia water to soften the blocks before cutting? If so, does this have any effect  on IHC staining? Any help will be greatly appreciated. Thanks.

Sharon Davis-Devine, CT (ASCP)
Cytology-Histology  Supervisor
Carle Foundation Hospital
Laboratory and Pathology Services
611 West Park Street
Urbana, Illinois 61801
217-383-3572
sharon.davis-devine <@t> carle.com

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