[Histonet] Re: Fluorescent dyes and xylene

[Bader Siddiki]

Hello
My experience is that most Immunofluorescence dyes do not withstand alcohol
and organic solvents dehydration.
You can use aqueous mounting medium with anti-fading agent.
Frozen brain tissue is tough to work with;  since it contains tons of
lipids.
Lots of mounting mediums will produce air bubbles because aqueous mounting
medium being hydrophilic and brain tissue being hydrophobic.
We are in the process of developing an aqueous mounting medium with
anti-fading agent and should be OK to use with tissue like brain, if
interested please let us know we can send you a sample for evaluation.
Bader

On Mon, Jul 20, 2009 at 11:22 AM, Bob Nienhuis <bob.nienhuis <@t> gmail.com>wrote:

> Sorry, I should have mentioned that this will be done on thick frozen
> sections.
>
> Bob
>
> On Mon, Jul 20, 2009 at 10:37 AM, Bob Nienhuis <bob.nienhuis <@t> gmail.com
> >wrote:
>
> > Will the Alexa Fluor dyes withstand some dehydration and clearing in
> > alcohol and xylene?
> >
> > If they won't are there any bright fluorescent dyes that can be obtained
> > conjugated to a secondary antibody that will?
> >
> > Looking to immunostain tyrosine hydroxylase in rodent brain with
> > a fluorescent marker in tissue that needs to be dehydrated and
> > cleared, perhaps with an abbreviated series.
> >
> > Bob Nienhuis
> > VA / UCLA Neurobiology Research
> >
> >
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
If any Q's please feel free to contact us
Have a nice day/weekend
Mit freundlichen Grüßen / With Kind Regards /
avec l'aimable ce qui concerne /
種とについて
Bader
Bader B Siddiki, PhD
Executive director,
Research and development
ImmunoBioScience corp.
Phone: + 425 367 4601
Phone: + 425 514 3761
Fax: + 425 367 4817
cell (mobile) phone: + 425 314 0199
e-mail address: baderbo <@t> gmail.com
Web site: www.immunobioscience.com
Marketing: phone: + 650 343 IBSC (4272)
               E-mail: anitaIBSC <@t> aol.com