[Histonet] Fluorescent dyes and xylene

[Merced M Leiker]

So you are saying that you cannot just mount the brain tissue in aqueous 
mounting medium at the end (after washing the excess secondary antibody)? 
You must dehydrate and clear before mounting (in a permament medium)?  Just 
curious...

Merced

--On Monday, July 20, 2009 10:37 AM -0700 Bob Nienhuis 
<bob.nienhuis <@t> gmail.com> wrote:

> Will the Alexa Fluor dyes withstand some dehydration and clearing in
> alcohol and xylene?
>
> If they won't are there any bright fluorescent dyes that can be obtained
> congugated to a secondary antibody that will?
>
> Looking to immunostain tyrosine hydroxylase in rodent brain with
> a fluorescent marker in tissue that needs to be dehydrated and
> cleared, perhaps with an abbreviated series.
>
> Bob Nienhuis
> VA / UCLA Neurobiology Research
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Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY  14214
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

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