[Histonet] Re: Fluorescent dyes and xylene

[Bob Nienhuis]

Sorry, I should have mentioned that this will be done on thick frozen
sections.

Bob

On Mon, Jul 20, 2009 at 10:37 AM, Bob Nienhuis <bob.nienhuis <@t> gmail.com>wrote:

> Will the Alexa Fluor dyes withstand some dehydration and clearing in
> alcohol and xylene?
>
> If they won't are there any bright fluorescent dyes that can be obtained
> conjugated to a secondary antibody that will?
>
> Looking to immunostain tyrosine hydroxylase in rodent brain with
> a fluorescent marker in tissue that needs to be dehydrated and
> cleared, perhaps with an abbreviated series.
>
> Bob Nienhuis
> VA / UCLA Neurobiology Research
>
>