[Histonet] RE: murine eosinophil immunostaining

[Bryan Hewlett]

Hi Chris and Gayle,

BMK-13 certainly works well in both human and rat, I used it for those
purposes about 10 years ago.
It's definitely worth a try on mouse.

Bryan
----- Original Message -----
From: "C.M. van der Loos" <c.m.vanderloos <@t> amc.uva.nl>
To: <Histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, January 07, 2005 2:51 AM
Subject: [Histonet] RE: murine eosinophil immunostaining


> Hi Gayle,
>
> I have an antibody here against human eosinophils that stains like
> hell. The datasheet said nothing if other species are stained or not.
> Considering the huge staining intensity it seems worthwhile to test
> (with a mouse-on-mouse detection system): anti-Eosinophil Major Basic
> Protein, mouse monoclonal antibody clone BMK-13 (MonoSan 6008, see:
> www.monosan.com). The antibody stains cryostat sections, smears and
> FFPE's (after pepsin pretreatment).
>
> Chris van der Loos, PhD
> Dept. of Pathology
> Academical Medical Center M2-230
> Meibergdreef 9
> NL-1105 AZ Amsterdam
> The Netherlands
>
> phone:  +31 20 5665631
> fax:    +31 20 6960389
> e-mail: c.m.vanderloos <@t> amc.uva.nl
>
> ----- Original Message -----
> >From  Gayle Callis <gcallis <@t> montana.edu>
> Date  Thu, 06 Jan 2005 09:56:29 -0700
> To  Histonet <@t> lists.utsouthwestern.edu
> Subject  [Histonet] murine eosinophil immunostaining
>
>
> We are embarking on murine eosinophil staining on FFPE fixed lung (not
> short fixation time!).  I will be doing other searches for available
> antibodies to detect eosinophils, but would like any recommendations
> from
> laboratories successfully doing murine eosinophil IHC.
>
> 1.  Whether this will work on FFPE tissue, if so, retrieval?
>
> 2.  Are frozen sections a preference - something I would prefer to do
> anyway.
>
> 3. Antibody source
>
> Any other suggestions will be welcome as this is NOT a project that
> originated in our laboratory so we have no control on fixation time in
> NBF.
> Gayle Callis
> MT,HT,HTL(ASCP)
> Research Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> PO Box 173610
> Bozeman MT 59717-3610
> 406 994-6367 (lab with voice mail)
> 406 994-4303 (FAX)
>
>
>
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