[Histonet] C4d on paraffins

[Brett, Lawrence]

We're using the Quindel ab' (product code A213) with Powervision detection
and EDTA/NaOH pH8.0 presssure cooking - (for some reason our usual Tris-EDTA
didn't work). Dilution of the primary is 1:200, with 30 minute incubation at
room temp. Good results.
 
In my hands, one seems to need a very sensitive detection system for this
ab', Powervision or Biogenex Super sensitive polymer kit will do the trick,
don't expect decent results with the more popular polymer detection kits.
 
Lawrence Brett
 
Royal Infirmary,
Edinburgh
Scotland, UK


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