[Histonet] RE: murine eosinophil immunostaining

[C.M. van der Loos]

Hi Gayle,

I have an antibody here against human eosinophils that stains like 
hell. The datasheet said nothing if other species are stained or not. 
Considering the huge staining intensity it seems worthwhile to test 
(with a mouse-on-mouse detection system): anti-Eosinophil Major Basic 
Protein, mouse monoclonal antibody clone BMK-13 (MonoSan 6008, see: 
www.monosan.com). The antibody stains cryostat sections, smears and 
FFPE's (after pepsin pretreatment).

Chris van der Loos, PhD
Dept. of Pathology
Academical Medical Center M2-230
Meibergdreef 9
NL-1105 AZ Amsterdam
The Netherlands

phone:  +31 20 5665631
fax:    +31 20 6960389
e-mail: c.m.vanderloos <@t> amc.uva.nl

----- Original Message ----- 
>From  Gayle Callis <gcallis <@t> montana.edu> 
Date  Thu, 06 Jan 2005 09:56:29 -0700 
To  Histonet <@t> lists.utsouthwestern.edu 
Subject  [Histonet] murine eosinophil immunostaining 


We are embarking on murine eosinophil staining on FFPE fixed lung (not 
short fixation time!).  I will be doing other searches for available 
antibodies to detect eosinophils, but would like any recommendations 
from 
laboratories successfully doing murine eosinophil IHC.

1.  Whether this will work on FFPE tissue, if so, retrieval?

2.  Are frozen sections a preference - something I would prefer to do 
anyway.

3. Antibody source

Any other suggestions will be welcome as this is NOT a project that 
originated in our laboratory so we have no control on fixation time in 
NBF.
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)