[Histonet] Brucella staining

[Philip Oshel]

Histomavens,

I've got a project coming in to look at bacteria in the SEM. No 
problem, I do that routinely. But, it seems to me that this group 
would be better off sectioning their samples and staining the 
bacteria.
Except they claim the bugs won't stain.
This is for Brucella melitensis in mouse tails.
Anyone have a good stain for these bacteria? The stain would be done 
on sections.
Calcified or decalified better? I don't think that matters to the 
study, but it might to the staining.
Thanks!

Phil
-- 
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison,  WI  53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)