[Histonet] Brucella staining

[Laurie Reilly]

Phil and all,
The Putt Modification of the Zeihl-Neelsen Stain is reputed to stain bovine 
Brucella.
The method is a cold ZN utilising New Fuchsin at room temperature and then 
treating with Saturated Aqueous Lithium Carbonate.
The method is in "Animal Tissue Techniques" by Gretchen L. Humason.

         Regards,  Laurie.

At 10:13 AM 03/11/04 -0600, Philip Oshel wrote:
>Histomavens,
>
>I've got a project coming in to look at bacteria in the SEM. No problem, I 
>do that routinely. But, it seems to me that this group would be better off 
>sectioning their samples and staining the bacteria.
>Except they claim the bugs won't stain.
>This is for Brucella melitensis in mouse tails.
>Anyone have a good stain for these bacteria? The stain would be done on 
>sections.
>Calcified or decalified better? I don't think that matters to the study, 
>but it might to the staining.
>Thanks!
>
>Phil
>--
>Philip Oshel
>Supervisor, BBPIC microscopy facility
>Department of Animal Sciences
>University of Wisconsin
>1675 Observatory Drive
>Madison,  WI  53706 - 1284
>voice: (608) 263-4162
>fax: (608) 262-5157 (dept. fax)
>
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Mr.Laurie Reilly                                              Ph 07 4781 4468
Physiology & Pharmacology                           Fax  07 4779  1526
Aust.Inst.of Tropical Vet.& Animal Sc.
James Cook University
Townsville  Qld. 
4811                                      laurie.reilly <@t> jcu.edu.au 

Australia.