[Histonet] IHC- using IgM vs. IgG

[Morken, Tim - Labvision]

Majid asks:  "if your primary antibody is a mouse IgM, by using a secondary
anti-mouse IgG are you running any risk  of getting artefactual signals?"
 
 
Yes, your results will be negative. The anti-IgG will not detect the IgM
primary antibody.
 
Tim Morken
Lab Vision / NeoMarkers
Fremont, CA 
www.labvision.com
 
-----Original Message-----
From: Majid Ghoddusi [mailto:mghoddusi <@t> cmri.usyd.edu.au] 
Sent: Monday, August 25, 2003 12:21 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] IHC- using IgM vs. IgG
 
Hello dear histonetters
 
Just a very basic question for IHC experts:
 
if your primary antibody is a mouse IgM, by using a secondary anti-mouse IgG
are you running any risk  of getting artefactual signals?
 
Thanks very much in advance for any help and advice including pointing to a
reference that might discuss this in detail.
 
 
 
...........................................................
Majid Ghoddusi 
Children's Medical Research Institute
Locked Bag 23, Wentworthville NSW 2145
Tel: (02) 9687-2800
Fax:(02) 9687-2120
.............................................................
 
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