[Histonet] cyclin-D1

[Sebree Linda A.]

Hi Heather,

Our protocol for Cyclin D1 (Neomarkers #MS-210-P, clone DCS-6) on our Ventana Benchmark is: CC1, P3/4", 1:50 for a 32" incubation. On our Ventana NexES our protocol is: 2" HIER in Biocare's decloaking chamber in Biocare's Nuclear Decloaking buffer, P3/8", 1:50 for a 24" incubation, Ventana amplification. Hope this helps; if you have any further questions, feel free to contact us.

Linda A. Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
A4/204-2472
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174


-----Original Message-----
From: Mcleod [mailto:hmcleod <@t> chempath.uct.ac.za]
Sent: Monday, August 25, 2003 8:35 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] cyclin-D1


Dear All

What is the preferred AR method for Cyclin-D1's?  We have the Dako (cat
no M7155) clone DCS-6 antibody and while it works well as a marker for
the cell cycle we have not had any success with Mantle Cell Lymphomas. 
We have tried the "standard" AR methods i.e. HIER at pH6 and pH.8 in a
pressure cooker at full power for 2 mins. as well as protease on our
Ventana Nexes system. Any help and suggestions very welcome.  

Thanks a stack in advance
Heather.

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