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<p class=MsoNormal style='margin-left:.5in'><span class=SpellE><font size=2
color=navy face=Arial><span style='font-size:10.0pt;font-family:Arial;
color:navy'>Majid</span></font></span><font size=2 color=navy face=Arial><span
style='font-size:10.0pt;font-family:Arial;color:navy'> asks:<span
style='mso-spacerun:yes'> </span>"</span></font><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'>if your primary antibody is a mouse <span
class=SpellE>IgM</span>, by using a secondary anti-mouse <span class=SpellE>IgG</span>
are you running any risk<span class=GramE> of</span> getting <span
class=SpellE>artefactual</span> signals?</span></font>"<o:p></o:p></p>
<p class=MsoNormal style='margin-left:.5in'><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'><o:p> </o:p></span></font></p>
<p class=MsoNormal style='margin-left:.5in'><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'><o:p> </o:p></span></font></p>
<p class=MsoNormal style='margin-left:.5in'><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'>Yes, your results will be negative. The anti-<span
class=SpellE>IgG</span> will not detect the <span class=SpellE>IgM</span>
primary antibody.<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'><o:p> </o:p></span></font></p>
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<p class=MsoAutoSig><font size=3 color=navy face="Courier New"><span
style='font-size:12.0pt;font-family:"Courier New";color:navy;mso-no-proof:yes'>Tim
Morken<o:p></o:p></span></font></p>
<p class=MsoAutoSig><font size=3 color=navy face="Courier New"><span
style='font-size:12.0pt;font-family:"Courier New";color:navy;mso-no-proof:yes'>Lab
Vision / NeoMarkers<o:p></o:p></span></font></p>
<p class=MsoAutoSig><st1:place><st1:City><font size=3 color=navy
face="Courier New"><span style='font-size:12.0pt;font-family:"Courier New";
color:navy;mso-no-proof:yes'>Fremont</span></font></st1:City><font
color=navy face="Courier New"><span style='font-family:"Courier New";
color:navy;mso-no-proof:yes'>, </span></font><st1:State><font color=navy
face="Courier New"><span style='font-family:"Courier New";color:navy;
mso-no-proof:yes'>CA</span></font></st1:State></st1:place><font color=navy
face="Courier New"><span style='font-family:"Courier New";color:navy;
mso-no-proof:yes'> <o:p></o:p></span></font></p>
<p class=MsoAutoSig><font size=3 color=navy face="Courier New"><span
style='font-size:12.0pt;font-family:"Courier New";color:navy;mso-no-proof:yes'>www.labvision.com<o:p></o:p></span></font></p>
</div>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'><o:p> </o:p></span></font></p>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face=Tahoma><span
style='font-size:10.0pt;font-family:Tahoma'>-----Original Message-----<br>
<b><span style='font-weight:bold'>From:</span></b> Majid Ghoddusi
[mailto:mghoddusi@cmri.usyd.edu.au] <br>
<b><span style='font-weight:bold'>Sent:</span></b> Monday, August 25, 2003
12:21 AM<br>
<b><span style='font-weight:bold'>To:</span></b> 'histonet@lists.utsouthwestern.edu'<br>
<b><span style='font-weight:bold'>Subject:</span></b> [Histonet] IHC- using IgM
vs. IgG</span></font></p>
<p class=MsoNormal style='margin-left:.5in'><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'><o:p> </o:p></span></font></p>
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<p class=MsoNormal style='margin-left:.5in'><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'>Hello dear histonetters</span></font><o:p></o:p></p>
</div>
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<p class=MsoNormal style='margin-left:.5in'><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
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<p class=MsoNormal style='margin-left:.5in'><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'>Just a very basic question for IHC
experts:</span></font><o:p></o:p></p>
</div>
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<p class=MsoNormal style='margin-left:.5in'><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
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<div>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'>if your primary antibody is a mouse
IgM, by using a secondary anti-mouse IgG are you running any risk of
getting artefactual signals?</span></font><o:p></o:p></p>
</div>
<div>
<p class=MsoNormal style='margin-left:.5in'><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
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<div>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'>Thanks very much in advance for any
help and advice including pointing to a reference that might discuss this
in detail.</span></font><o:p></o:p></p>
</div>
<div>
<p class=MsoNormal style='margin-left:.5in'><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'> </span></font><o:p></o:p></p>
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<p class=MsoNormal style='margin-left:.5in'><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
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<p class=MsoNormal style='margin-left:.5in'><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'>...........................................................</span></font><o:p></o:p></p>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'>Majid Ghoddusi </span></font><o:p></o:p></p>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'>Children's Medical Research
Institute</span></font><o:p></o:p></p>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'>Locked Bag 23, Wentworthville NSW 2145</span></font><o:p></o:p></p>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'>Tel: (02) 9687-2800</span></font><o:p></o:p></p>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'>Fax:(02) 9687-2120</span></font><o:p></o:p></p>
<p class=MsoNormal style='margin-left:.5in'><font size=2 face=Arial><span
style='font-size:10.0pt;font-family:Arial'>.............................................................</span></font><o:p></o:p></p>
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<p class=MsoNormal style='margin-left:.5in'><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'> <o:p></o:p></span></font></p>
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