[Histonet] Performing IHC stain on an H&E stained slides

[jdhannasch at gmail.com]

I believe the ventana stainer does the antigen retrieval step as part of their protocol. If someone is staining by hand or has a stainer that does not do the antigen retrieval step, they will need to make sure to do it before staining. 

> On Sep 24, 2025, at 7:45 AM, Histology <histo at pathlab.us> wrote:
> 
> We do this often.  We take the coverslip off and put the slide in xylene for at least 5 minutes.  Then, we run it down to water.  From the water, we load it on our Ventana ultra with the same protocol as normal but without the deparaffination step.  They have an option for "wet-load".  I'm sure this can be done on other platforms and manually as well.
> 
> 
> Mehndi Helgren
> Lab Manager
> 757-664-7901
> Dominion Pathology Labs.
> 733 Boush St. Suite 200
> Norfolk, VA  23510
> 
> 
> 
> -----Original Message-----
> From: ADESUPO ADESUYI via Histonet <histonet at lists.utsouthwestern.edu>
> Sent: Tuesday, September 23, 2025 1:40 PM
> To: histonet at lists.utsouthwestern.edu
> Subject: [Histonet] Performing IHC stain on an H&E stained slides
> 
> Hi,
> 
> Does anyone has a protocol for performing IHC stain on an H&E stained slides to share?
> 
> Sincerely,
> Ade
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