[Histonet] Performing IHC stain on an H&E stained slides

[Histology]

We do this often.  We take the coverslip off and put the slide in xylene for at least 5 minutes.  Then, we run it down to water.  From the water, we load it on our Ventana ultra with the same protocol as normal but without the deparaffination step.  They have an option for "wet-load".  I'm sure this can be done on other platforms and manually as well.


Mehndi Helgren
Lab Manager 
757-664-7901
Dominion Pathology Labs.
733 Boush St. Suite 200
Norfolk, VA  23510



-----Original Message-----
From: ADESUPO ADESUYI via Histonet <histonet at lists.utsouthwestern.edu> 
Sent: Tuesday, September 23, 2025 1:40 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Performing IHC stain on an H&E stained slides

Hi,

Does anyone has a protocol for performing IHC stain on an H&E stained slides to share?

Sincerely,
Ade
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