[Histonet] Histonet Digest, Vol 258, Issue 2

[Amos Brooks]

Hi,
    I do not use this mounting media.we use a traditional xylene based
resin. We recoverslip very often. Sometimes it is a poorly coverslipped
slide or one that ended up with two coverslips on the slide. That happens
occasionally.
     More frequently however is that there is a request to have another
stain on a specific slide. So, the coverslip would be removed and either
the prior stain is destained or simply stained over the prior one. In the
interest of not wasting tissue from the block or when a block is not
available due to being either exhausted, sent out to another institution or
simply misplaced this is needed on occasion.
     I would find an inability to remove a coverslip an extreme red flag
when it comes to a choice of coverslipping methods. Even if you rarely need
this, the one time you do makes the inability to do so a huge problem. For
my lab, I would never paint myself into a corner by using a product that
eliminates possible downstream applications.

Amos Brooks

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>
> Message: 1
> Date: Thu, 1 May 2025 10:29:21 -0700
> From: John O?Brien <john at imebinc.com>
> To: histonet at lists.utsouthwestern.edu
> Subject: Re: [Histonet] Histonet Digest, Vol 258, Issue 1
> Message-ID: <6014A8CA-3161-48C0-ABAF-6BF31749693B at imebinc.com>
> Content-Type: text/plain; charset=utf-8
>
> Hi Mr. Martin
> Thank you for responce,
> I am a supplier of pathology supplies and instruments and we keep getting
> asked can our coverslipped slides be removed and re stained and
> recoverslipped, we have developed  mounting media that is xylene free and
> can coverslip straight out of alcohol or coverslip dry ,
> It?s a special plastic mix that dries in 30 seconds with UV light,
> Problem is it?s difficult to remove coverslip cleanly thus trying
> determine % of slides to be recoverslipped would help with understanding
> how it is big or small issue
> Any help is appreciated
> Regards
> John
> IMEB
>