[Histonet] Histonet Digest, Vol 258, Issue 1
John O’Brien
john at imebinc.com
Thu May 1 12:29:21 CDT 2025
Hi Mr. Martin
Thank you for responce,
I am a supplier of pathology supplies and instruments and we keep getting asked can our coverslipped slides be removed and re stained and recoverslipped, we have developed mounting media that is xylene free and can coverslip straight out of alcohol or coverslip dry ,
It’s a special plastic mix that dries in 30 seconds with UV light,
Problem is it’s difficult to remove coverslip cleanly thus trying determine % of slides to be recoverslipped would help with understanding how it is big or small issue
Any help is appreciated
Regards
John
IMEB
> On May 1, 2025, at 10:13 AM, histonet-request at lists.utsouthwestern.edu wrote:
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> Today's Topics:
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> 1. Re: Histonet Digest, Vol 257, Issue 11 (Eddie Martin)
> 2. Re: Histonet Digest, Vol 257, Issue 10 (Eddie Martin)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Thu, 1 May 2025 12:54:07 -0400
> From: Eddie Martin <edmartin26 at gmail.com>
> To: histonet at lists.utsouthwestern.edu
> Subject: Re: [Histonet] Histonet Digest, Vol 257, Issue 11
> Message-ID:
> <CADpZgVTyS6J5bos9Mx1Rn6jZ_pXij_-q2du2xpaW_tj1jgydHw at mail.gmail.com>
> Content-Type: text/plain; charset="UTF-8"
>
> Hi John,
>
> A few common scenarios of why recoverslipping would be needed may give you
> the better response you're searching for. An example may be that the lab
> is diluting their coverslip medium too much and slides look great for a few
> days, or another scenario, where they forget to prime the tubing in their
> instruments or the viscosity is too thick, causing air to get
> dispensed rather than coverslipping medium.
>
> Instead of a re-coverslipping percentage, what information are you looking
> for?
>
> Best wishes.
>
> Eddie Martin
> National Institutes of Health
>
>
>> On Tue, Apr 29, 2025 at 1:00?PM <histonet-request at lists.utsouthwestern.edu>
>> wrote:
>>
>> Send Histonet mailing list submissions to
>> histonet at lists.utsouthwestern.edu
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>> To subscribe or unsubscribe via the World Wide Web, visit
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> or, via email, send a message with subject or body 'help' to
>> histonet-request at lists.utsouthwestern.edu
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>> When replying, please edit your Subject line so it is more specific
>> than "Re: Contents of Histonet digest..."
>> Today's Topics:
>>
>> 1. Removing H&E coverslippped slide (John O?Brien)
>>
>>
>>
>> ---------- Forwarded message ----------
>> From: "John O?Brien" <john at imebinc.com>
>> To: histonet at lists.utsouthwestern.edu
>> Cc:
>> Bcc:
>> Date: Mon, 28 Apr 2025 11:50:40 -0700
>> Subject: [Histonet] Removing H&E coverslippped slide
>>
>> Histonetters
>> Can you experts in staining and Coverslipping offer the number of times
>> or % of slides recover slipped to a differ stain, any opinion is
>> appreciated
>> John
>> IMEB Inc
>>
>>
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> ------------------------------
>
> Message: 2
> Date: Thu, 1 May 2025 12:57:18 -0400
> From: Eddie Martin <edmartin26 at gmail.com>
> To: histonet at lists.utsouthwestern.edu
> Subject: Re: [Histonet] Histonet Digest, Vol 257, Issue 10
> Message-ID:
> <CADpZgVS=2ua+0ka8tNEkEay04LvHscS-zv4n+GdeTRu46D7R4w at mail.gmail.com>
> Content-Type: text/plain; charset="UTF-8"
>
> Carl,
>
> That's really cool that Histonet was storing histology images. Sad to
> hear this after the fact. I'm wondering what kind of histology images?
> Were format were you saving them?
>
> Thanks,
> Eddie Martin
>
>
>
> On Sun, Apr 20, 2025 at 1:00?PM <histonet-request at lists.utsouthwestern.edu>
> wrote:
>
>> Send Histonet mailing list submissions to
>> histonet at lists.utsouthwestern.edu
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>> or, via email, send a message with subject or body 'help' to
>> histonet-request at lists.utsouthwestern.edu
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>> When replying, please edit your Subject line so it is more specific
>> than "Re: Contents of Histonet digest..."
>> Today's Topics:
>>
>> 1. Histology images (Carl Hobbs)
>>
>>
>>
>> ---------- Forwarded message ----------
>> From: Carl Hobbs <carl.hobbs at kcl.ac.uk>
>> To: histonet <histonet at lists.utsouthwestern.edu>
>> Cc:
>> Bcc:
>> Date: Sat, 19 Apr 2025 17:53:33 +0000
>> Subject: [Histonet] Histology images
>> Hi
>> I used to upload my Histology images to Histonet Images archive
>> I have been informed that this no longer exists: hence my images are
>> "LOST"
>> I'd be grateful if somebody would suggest where best to upload my images,
>> to share?
>>
>> It's good to share-ily
>> Carl
>>
>>
>> Carl Hobbs FIBMS
>> Histology and Imaging Manager
>> Wolfson SPaRC
>> Guys Campus, London Bridge
>> Kings College London
>> London
>>
>>
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