[Histonet] Copper Staining

[Tony Henwood]

We have found issues with rhodanine staining:

A common problem with this stain is the gradual fading of rhodanine staining over several days, particularly in biopsy specimens from patients with Primary Biliary Cirrhosis and when copper is scanty (Jain et al 1978 Journal of clinical pathology, 31(8), 784-790). A similar problem was noted by Lindquist (1969, Archives of pathology, 87(4), 370-379), using Permount as a mounting medium for the sections. Jain et al (1978 Journal of clinical pathology, 31(8), 784-790) found that the degree of fading differed according to the mounting medium used. This was greatest with Eukitt, less with DPX and Diatex, and least with Ralmount (all products of Raymond A. Lamb). We found that aqueous mountants such as Aquamount preserved staining.



Regards,



Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired)

Principal Scientist, the Children’s Hospital at Westmead (Retired)

Adjunct Fellow, School of Medicine, University of Western Sydney.

________________________________
From: Ni Nie via Histonet <histonet at lists.utsouthwestern.edu>
Sent: 15 April 2025 01:03
To: Histonet at lists.utsouthwestern.edu <Histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Copper Staining

Does anyone have any tricks for Copper staining?  Our techs always struggle
with it. Sometimes it looks good right after staining, but it fades after a
few days. Other times after lengthy staining we just don't get good results.
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